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Metaphase - is perhaps the most recognizable phase of mitosis, in which duplicate chromosomes align at the center of the cell, sometimes referred to as the metaphase plate. The alignment of chromosomes is facilitated by spindle microtubules, which apply traction to chromosome pairs via their kinetochores.
Metaphaseis particulary useful in cytogenetics, because chromosomes can be most easily visualized at this stage.Furthermore, cells can be experimentally arrested at Metaphase with mitotic poisons such as colchicine.Video microscopy shows that chromosomes temporarily stop moving during Metaphase
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