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Biuret reaction for the analysis of serum protein depends on

Sagot :

The Biuret reaction test is a type of analysis that determine the presence of the peptide bonds in a certain serum. This type of test depends on the number of peptide bonds that can be seen on the substance.

The reagent used for the Biuret Test are:

  • Copper sulfate (CuSO40)
  • Sodium hydroxide (NaOH)
  • Sodium potássium tartarate

For the interpretation of data, a serum is said to be positive for the presence of peptide bonds if the solution change from color blue to deep purple.

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