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The production of testosterone in the interstitial cells was stimulated by the Lutenizing Hormone (LH) or Interstitial cell-stimulating hormone (ICSH).
The LH was produced in the anterior pituitary gland and was triggered by the Gonadotropin-releasing hormone (GnRH). The target tissue of the LH is the interstitial cells within the testes. When the LH stimulates the testosterone, the hormones per se has a stimulatory effects on the seminiferous tubules which is essential on the development of reproductive organs and the secondary sexual characteristics of males.