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Answer:
All genetic engineering processes involve the modification of DNA. Traditionally DNA was isolated from the cells of organisms. Later, genes came to be cloned from a DNA segment after the creation of a DNA library or artificially synthesised. Once isolated, additional genetic elements are added to the gene to allow it to be expressed in the host organism and to aid selection.
Explanation:
Commonly used methods include:
✓microinjection of DNA into the nucleus of anchored cells;
✓electroporation, where DNA is introduced through cell membrane pores by pulsed electrical charges;
✓polycationic neutralization of the cell membrane and the DNA to be introduced to improve passive uptake;