Sumali sa IDNStudy.com at makuha ang mga sagot ng eksperto. Ang aming mga eksperto ay handang magbigay ng malalim na sagot at praktikal na solusyon sa lahat ng iyong mga tanong.
Answer:
The size of inoculum does affect the MIC, but the effect depends on the antibiotic and its mechanism of action. There are two main reasons behind this:
1- Increased cell number result in decreased effective number of antibiotic molecules/cell (you may say per target molecule). Depending on the mechanism of action it may change kinetics of killing or prevent killing altogether.
2- Increased inoculum results in fewer cell generations before culture reaches saturation. Some antibiotics require active growth for killing (e.g. beta-lactams) and it takes few generations to accumulate a defect which would result in cell death. Hence, if you start with the dense culture you may not have enough time (number of cell divisions) to observe the killing.
Unfortunately, the MIC change isn't just simply proportional to the cell number, and there is no way of calculating it. That's why the MIC determination protocols are standardized for 10^5 cell/ml, so it can be directly comparable.
Explanation: