Direction: Arrange the following procedures in running an autoclave by
writing a letter before each number.

___1. The drainage pipe is then closed, and the steam inside is allowed
to reach the desired levels (15 lbs in most cases).

___2. Now, the electric heater is switched off, and the autoclave is al-
lowed to cool until the pressure gauge indicates the pressure inside has low-
ered down to that of the atmospheric pressure.

___3. Finally, the lid is opened, and the sterilized materials are taken
out of the chamber.

___4. Now, the materials to be sterilized are placed inside the chamber.

___5. Before beginning to use the autoclave, it should be checked for
any items left from the previous cycle.

___6. The safety valves are adjusted to maintain the required pressure
in the chamber.

___7. After the whistle, the autoclave is run for a holding period, which
is 15 minutes in most cases.

___8. A sufficient amount of water is then put inside the chamber.

___9. The discharge pipe is then opened to allow the entry of air from
the outside into the autoclave.

___10. Once the pressure is reached, the whistle blows to remove ex-
cess pressure from the chamber.

___11. The lid is then closed, and the screws are tightened to ensure an
airtight condition, and the electric heater is switched on.

___12. Once the water inside the chamber boils, the air-water mixture
is allowed to escape through the discharge tube to let all the air inside to be
displaced. The complete displacement can be ensured once the water bubbles
cease to come out from the pipe.
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