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Complete the following tasks.
You discovered that a species of bacteria can break down Styrofoamâ„¢ (polystyrene) products due to an
enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme.
Task 1: DNA Extraction
To begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose of
the following procedures? Answer briefly but completely.
a. Using sodium dodecyl sulfate, a detergent
Answer: __________________________________
b. Adding RNase A and Proteinase K during extraction
Answer: __________________________________
c. Adding ethanol before recovering the DNA extract
Answer: __________________________________